Essay Archive - Determination of An Unknown Amino Acid From Titration
Determination of An Unknown Amino Acid From Titration
Experiment 11 used a titration curve to determine the identity of an
unknown amino acid. The initial pH of the solution was 1.96, and the pKa’s
found experimentally were 2.0, 4.0, and 9.85. The accepted pKa values were
found to be 2.10, 4.07, and 9.47. The molecular weight was calculated to be
176.3 while the accepted value was found to be 183.5. The identity of the
unknown amino acid was established to be glutamic acid, hydrochloride.
Introduction
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